Fig. 2

ERβ knockdown inhibited 25-HC-induced cell proliferation. A549 and SPC-A1 cells were transfected with the lentivirus carrying ctrl-sgRNA or ESR2-sgRNA, and were then exposed to 0, 0.013 and 0.085 µM 25-HC for 48 h. Cell proliferation was determined by CCK8 assay (A) and Edu proliferation assay (B). Cell necrosis and apoptosis were determined by flow cytometry analysis. Data were shown as mean ± SD of at least three independent experiments.*, p < 0.05; **, p < 0.01;*** p < 0.0001