Fig. 5
From: UBE2C promotes the proliferation of acute myeloid leukemia cells through PI3K/AKT activation
UBE2C affects proliferation and ferroptosis in leukemic cells by regulating the PI3K/AKT pathway. (A) The WB analysis detects the expression levels of p-PI3K, PI3K, and p-AKT, AKT proteins in HL60 and THP-1 cells. (B) The CCK-8 method detects the viability of HL60 and THP-1 cells after incubation for 0, 24, 48, and 72 h. (C) Flow cytometry detects the apoptosis levels of HL60 and THP-1 cells after different treatments. (D) The iron assay kit determines Fe2+ levels in HL60 and THP-1 cells. 740 Y-P was applied at 5 µM. ** signifies P < 0.01 compared with the sh-NC treatment group; *** indicates P < 0.001 compared with the sh-NC treatment group; # represents P < 0.05 compared with the sh-NC + 740 Y-P treatment group; ## presents P < 0.01 compared with the sh-NC + 740 Y-P treatment group