Fig. 4

The number of apoptotic naive (nCD8⁺T-cells), trained naive (trained nCD8⁺T-cells), trained and exhausted naive (trained and exhausted nCD8⁺T-cells), reprogrammed (rCD8⁺T-cells), and exhausted reprogrammed (exhausted rCD8⁺T-cells) CD8⁺ T-cells isolated from the spleen of C57BL/6 mice after co-culturing with LLC (% from added cells) in ratio between T-lymphocytes and LLC 0.25:1, 1:1, 2.5:1, 5:1, and 10:1. *– for comparison with the naive T-cells, ■– for comparison with trained naive T-cells, ▲– for comparison with reprogrammed T-cells (Mann–Whitney test, p-value < 0.05). Data were obtained by analyzing images obtained using Cytation 5 Cell Imaging Multimode Reader, widefield fluorescence microscopy