Fig. 3

The number of CCR7⁺ T-cells in a culture of naive (nCD8⁺T-cells), trained naive (trained nCD8⁺T-cells), trained and exhausted naive (trained and exhausted nCD8⁺T-cells), reprogrammed (rCD8⁺T-cells), and exhausted reprogrammed (exhausted rCD8⁺T-cells) CD8⁺ T-cells isolated from the spleen of C57BL/6 mice. A The count of nCD8⁺T-cells, trained nCD8⁺T-cells, trained and exhausted nCD8⁺T-cells, rCD8⁺T-cells, and exhausted rCD8⁺T-cells of the spleen of C57BL/6 mice expressing the CCR7 marker in T-cells culture. Data were obtained by analyzing images obtained using Cytation 5 Cell Imaging Multimode Reader, widefield fluorescence microscopy.B 4× images of T-cells stained with: Hoechst 34,580 (blue) to identify cell nuclei; CD8 FITC (green); CCR7 AF555 (red); (Hoechst⁺CD8⁺ CCR7⁺) composite image using all three colors. Determination of the percentage of cells CD8⁺CCR7⁺ is made by the ratio of cells counted in green and red channel to total cells counted using blue (DAPI) channel. Images were obtained using Cytation 5 Cell Imaging Multimode Reader, widefield fluorescence microscopy. All scale bars are 1000 μm. *– for comparison with the naive T-cells, ■– for comparison with trained naive T-cells (Mann–Whitney test, p-value < 0.05)