Fig. 2
From: CircPHGDH downregulation decreases papillary thyroid cancer progression through miR-122-5p/PKM2 axis
Downregulation circPHGDH suppressed PTC proliferation and tumor growth in both in vitro and in vivo experiment. (A) RT-qPCR detection show the expression of circPHGDH in both SW579 and TPC-1 cells after transfected with NC or si-circPHGDH. The data are expressed as the mean ± SD. ***p < 0.001 vs. NC. (B and C) CCK8 detection show the proliferation ability of SW579 and TPC-1 cells. The data are expressed as the mean ± SD. ***p < 0.001 vs. NC. (D and E) EdU assay show the proliferation of both SW579 and TPC-1 cells after transfected with NC or si-circPHGDH. The data are expressed as the mean ± SD. ***p < 0.001 vs. NC. Scale bar, 50 μm. (F) Representative photographs of TPC-1 tumor formation in the xenografts of nude mice. (G) Summary of the tumor volume in mice that were measured every five days. Data are presented as the mean ± SD. ***p < 0.001 vs. NC. (H) Tumor weight was measured at 25 days post-injection. Data are presented as the mean ± SD. ***p < 0.001 vs. NC. (I and J) Immunohistochemical analysis showing the percentage of Ki67-positive cells. Data are presented as the mean ± SD. ***p < 0.001 vs. NC. Scale bar, 50 μm