Fig. 3

Down-regulated HHLA2 was associated with CD8⁺CD103⁺TRM enrichment. (A) Comparison of negative regulation of immune response, T cell proliferation, and CD8⁺T cell activation gene sets from the MSigDB database based on TCGA in the HHLA2^low and HHLA2^high group. (B) Representative flow cytometry chart of CD8⁺CD103⁺TRM in the HHLA2^low versus HHLA2^high group. (C) Representative flow cytometry chart of GZMB, IFNG, PD1 among CD8⁺CD103⁺TRM in the HHLA2^low versus HHLA2^high group. (D) Statistical maps of CD8⁺CD103⁺TRM proportion and function in the HHLA2^low versus HHLA2^high group. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001