Fig. 4

Identification downstream target genes regulated by RTEL1. (A) Heatmap plot of differentially expressed genes in RTEL1-depleted U87 and SF297 cells and the common 1131 genes of these two cell lines was selected performed by Genenergy Bio-technology (Shanghai, Inc. https://www.genenergy.cn). (B) Phospho-specific antibody microarray was used and Fluorescence-based assay for screening of differentially expressed proteins in RTEL1-depleted U87 cells performed by Wayen Biotechnologies (Shanghai, Inc. https://www.wayenbio.com/). (C) Western blot analysis of phosphorylated ELK1 and the JNK signaling pathway in RTEL1-depleted U87 and SF297 cells with GAPDH as the normalized controls. si-N.C represents siRNA of non-target as controls; si-RTEL1-1724 represents siRNA of RTEL1.