Fig. 2

RTEL1 knockdown inhibits the malignant biological properties of glioma. Inhibition efficiency of the two different specific short interfering RNAs (siRNAs) targeting RTEL1 (si-RTEL1-1724 and − 3794) was determined by quantitative RT-PCR (qRT-PCR) (A) and Western blot assays (B) with 18 S rRNA and GAPDH as the normalized controls. (C) MTT assay was used to examine the effect of RTEL1 knockdown on glioma cell proliferation. RTEL1 knockdown significantly inhibited cell proliferation compared to the negative control. (D) The impact of RTEL1 knockdown on colony formation ability of glioma cells using soft agar. And RTEL1 knockdown significantly inhibited colony formation ability. (E) Cell migration and invasion assays were assessed by transwell chambers. And RTEL1 knockdown decreased migration and invasion potential of glioma cells. Mann–Whitney U test was used and data were expressed as mean ± SD. ** p < 0.01, *** p < 0.001