Fig. 5From: Biplex quantitative PCR to detect transcriptionally active human papillomavirus 16 from patient salivaBiplex detection of E6 and E7 mRNA in saliva specimens. RNA was isolated from the saliva of eight HNSCC patients (n = 3 p16 positive; n = 5 p16 negative). Negative experimental controls included cDNA (NTC) and PCR negative reactions. All reactions were completed in triplicate. A Beta 2 Microglobulin (B2M) was used as a positive control and reference gene to ensure expression observed in the E6 and E7 assays was positive and accurate, B E6 assay, C E7 assayBack to article page