Fig. 2From: Biplex quantitative PCR to detect transcriptionally active human papillomavirus 16 from patient salivaEvaluating total RNA input for E6 and E7 Single and Biplex reactions. AÂ E6 plex assay; BÂ E7 plex assay. Cq 35 was determined to be a reasonable threshold for a positive result. All reactions were completed in triplicate. PCR efficiencies for CÂ E6 assay; DÂ E7 at different total RNA inputs. 100% PCR efficiency is depicted at 2.0, with the acceptable threshold of efficiency at 1.5Back to article page