Fig. 5
The overexpression efficiency of CADM3 in MCF-7 and MDA-MB-231 cell lines verified by (A) WB and (B) qPCR. (C) Results of clonal formation assay. The cell colonies of the three groups were counted, and the data were presented as the mean values of three independent experiments (*p < 0.05, **p < 0.01, n = 3). (D) Results of CCK-8 experiment. CCK-8 was added to MCF-7 cells at 0, 24, 48, 72, and 96 h of cell adherence, while MDA-MB-231 cells were added to CCK-8 at 0, 12, 24, 36, and 48 h of cell adherence. Data showed the mean of three independent experiments (*p < 0.05, **p < 0.01, ****p < 0.0001, n = 3). (E) Results of cell scratch assay. Results of cell scratch assay. The scratch widths of the experimental group and the control group at 0, 12, 24 and 36 h were observed respectively, and the data were presented as the mean values of three independent experiments (**p < 0.01, ***p < 0.001, n = 3). (F) Results of transwell assay. Quantization map data showed the mean values of three independent experiments (*p < 0.05, n = 3)