Table 1 PCR primers
Cloning Primer | MCS Restriction site | Sequence |
|---|---|---|
N-Terminal-AR/ARV7-FW | Xhol | 5’-CTCGAGATGGAAGTGCAGTTAGGGCTGG-3’ |
N-Terminal-AR-RV | Bglll | 5’-AGATCTGCTTCACTGGGTGTGGAAATAGATGG-3’ |
N-Terminal-AR-V7-RV | Bglll | 5’-AGATCTTCTTCAGGGTCTGGTCATTTTGAGAT-3’ |
C-Terminal-AR/ARV7-FW | Bglll | 5’-AGATCTATGGAAGTGCAGTTAGGGCTGG-3’ |
C-Terminal-AR-RV | Xhol | 5’-CTCGAGCCCTGGGTGTGGAAATAGATGGGCTTG-3’ |
C-Terminal-AR-V7-RV | Xhol | 5’CTCGAGCCGGGTCTGGTCATTTTGAGATGCTTGCA-3’ |