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Fig. 2 | BMC Cancer

Fig. 2

From: NanoLuc Binary Technology as a methodological approach: an important new tool for studying the localization of androgen receptor and androgen receptor splice variant V7 homo and heterodimers

Fig. 2

Immunofluorescence staining of NanoLuc luciferase. HEK-293 cells were transfected with the indicated combinations of plasmid vectors. Immunofluorescence (IF) staining was performed using NanoLuc and Alexa Fluor 488 antibodies. IF staining showing the subcellular localization of the AR-FL and AR-V7 proteins before and after androgen stimulation. The indicated combination constructs were transfected into HEK-293 cells, and IF staining was conducted 48 h after transfection. The cells were stimulated with 1 nM DHT for 0, 15, 30 or 60 min. DAPI was used for nuclear staining. The scale bar represents 10 µm. Only merged pictures are shown

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BMC Cancer

ISSN: 1471-2407

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