Fig. 3

The tumor immune microenvironments of KPCML1 primary and metastatic lesions are conserved. A Representative photo of an excised liver with KPCML1 metastatic nodules. B Representative images of metastatic tumors lesions (T) and adjacent normal liver tissue (N; where indicated) stained by H&E, CK19, and SMA. C Digital spatial profiling of an immune-oncology panel comparing the primary and metastatic KPCML1 tumors; images on the left depict the selected regions of interest (ROIs) and on the right is a representative image of an ROI from the primary and metastatic KPCML1 tumor at higher magnification. D A cluster of immune markers were identified from the 12 selected ROIs and heat map profiles were generated and compared between the primary and metastatic tumor