Fig. 1

Generation and characterization of the KPCML1 cell line. A Representative phase contrast microscopic images of the KPC parental and KPCML1 metastatic cell lines, shown at two magnifications. B Graph depicting the growth of KPC and KPCML1 cell lines in culture over 4 days. C Volcano plot of DEGs from RNA-seq results comparing KPCML1 to KPC cells. Expression of Has1, Syt8, and Klk8 genes are indicated by arrows. D Heatmaps of significantly altered genes (p < 0.05) expressed from KPCML1 (n = 2) versus KPC cells. E Quantitative real-time PCR analyses of Has1, Syt8, and Klk8 genes in KPCML1 cells compared to KPC cells (n = 3). Data are shown as mean ± SEM. F Gene ontology analysis on differentially expressed genes from RNA-seq analysis of KPCML1 to KPC cells. * represents p < 0.0005, as determined by two tailed t tests with unequal variance