Fig. 5
From: Regulation of cancer stem cells by CXCL1, a chemokine whose secretion is controlled by MCM2
Regulation of GSC and EMT Properties by MCM2 Inhibition. (A) Expression of GSC marker proteins by MCM2 gene suppression in GSCs. Full-length blots are presented in Supplementary Fig. 1J. (B) Confirmation of marker protein expression in GSCs using ICC. (C) Comparison of differences in sphere formation according to the expression level of MCM2. (D) Comparison of expression of EMT marker proteins by MCM2 gene suppression. Full-length blots are presented in Supplementary Fig. 1K. (E) Confirmation of EMT marker proteins expression using ICC. (F) Analysis of cell migration and invasion ability after inhibiting the expression of MCM2. (G) Analysis of the regulation of CXCL1 expression by MCM2 in GSCs. Sample derive from the same experiment and that gels/blots were processed in parallel. Data are presented as the mean ± standard deviation of three replicates. Scale bar, 50 μm. *p˂0.001, **p˂0.005