Fig. 4

Regulation of Secreted Factors by MCM2 in LCSC. (A) Identification of secreted factors regulated by MCM2 using cytokine array. (B) Gene expression of secreted factors (CCL5, CXCL1, IL-18, IL-21) according to the expression level of MCM2. (C) Comparative analysis after conducting sphere-forming assay by treating with neutralizing antibodies of each secreted factor. (D) Comparative analysis of cell migration and invasion ability by treatment with neutralizing antibodies of each secreted factor. (E) Expression levels of CSC marker proteins after treatment with CXCL1 neutralizing antibody. Full-length blots are presented in Supplementary Fig. 1F. (F) Expression levels of EMT marker proteins after treatment with CXCL1 neutralizing antibody. Full-length blots are presented in Supplementary Fig. 1G. (G) Confirmation of the protein expression levels of MCM2 and CXCL1 after treatment with CXCL1 neutralizing antibody. Full-length blots are presented in Supplementary Fig. 1H. (H) Confirmation of gene expression levels of MCM2 and CXCL1 after treatment with CXCL1 neutralizing antibody. (I) Investigation of the CXCL1 signaling mechanism after inhibiting the action of CXCL1. Full-length blots are presented in Supplementary Fig. 1I. Sample derive from the same experiment and that gels/blots were processed in parallel. Data are presented as the mean ± standard deviation of three replicates. Scale bar, 50 μm. *p˂0.05, **p˂0.005, ***p˂0.0005