Fig. 5
From: Hsa_circ_0004872 alleviates meningioma progression by sponging miR-190a-3p/PTEN signaling
MiR-190a-3p inhibition acted as a tumor-suppressing effect by modulating PTEN/PI3K/AKT pathway. (A) IOMM-Lee and CH157-MN cells were transfected with inhibitor NC or miR-190a-3p inhibitor, and miR-190a-3p expression in cells was detected using qRT-PCR. IOMM‐Lee and CH157-MN cells were transfected with miR-190a-3p inhibitor or inhibitor NC combined with PTEN inhibitor (SF1670) treatment. (B) PTEN, PI3K, p-PI3K, AKT and p-AKT protein level were examined using western blot. (C) Cell viability was assessed using CCK8 assay. (D) Cell apoptosis was detected by flow cytometry. (E) Western blot was adopted to measure Bax, Bcl-2, Cleaved-caspase3, and Caspase3 protein levels. Data are presented as mean ± SD from at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001