Fig. 3

Hsa_circ_0004872 directly targets miR-190a-3p. (A) The subcellular localization of hsa_circ_0004872 was determined by qRT-PCR. (B) The potential binding site between hsa_circ_0004872 and miR-190a-3p. (C-D) The interaction between hsa_circ_0004872 and miR-190a-3p was assessed by dual-luciferase reporter gene and RIP assays. (E) MiR-190a-3p expression in IOMM-Lee and CH157-MN cells after pcDNA3.1 or pcDNA3.1-circ_0004872 transfection was measured by qRT-PCR. (F) MiR-190a-3p expression in tumor and adjacent tissues from meningioma patients was detected by qRT-PCR (n = 30). (G) Correlation analysis between hsa_circ_0004872 and miR-190a-3p expressions in clinical samples using Pearson’s correlation. (H) MiR-190a-3p expression in meningioma cells (IOMM‐Lee and CH157-MN) and human normal meningothelial cells (MECs) was determined by qRT-PCR. Data are presented as mean ± SD from at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001