Fig. 2

Suppressing TAGLN2 expression reduces cell proliferation, migration, and tumorigenic ability. (A) The effect of TAGLN2 suppression on the expression of EMT-associated proteins, including mesenchymal markers N-cadherin, Snail, and JAG2 and epithelial marker occludin. (B–D) TAGLN2 suppression by shRNA reduces (B) cell proliferation, (C) migration, and (D) invasion; ***, P < 0.001 shTAGLN2-1- and shTAGLN2-2-transfected vs. control vector-transfected (shCon) SNU1196 cells. (E) Injection of shTAGLN2-1, shTAGLN2-2 cells suppressing TAGLN2 expression into mice reduces the tumor mass in treated groups compared to the shCon group; n = 5 in each group, Data represents mean ± SEM. (F) The expression of Ki67, a cell proliferation marker in TAGLN2-suppressed mice tumor tissues