Fig. 7
Effect of combined treatment of oxaliplatin with Tig/Tet on the sensitivity of CRC cells. Equal numbers of cells were seeded in 96-well plates (10,000 cells/well) in regular media, and the next day, the media was replaced with media containing different combinations of agents as indicated for varying concentrations/time points. A Graphs represent the relative viability of cells treated with a different concentrations of oxaliplatin for 48h and b treated with 1µM of oxaliplatin for different time points. B Graphs represent the relative viability of the combined treatment of oxaliplatin with a Tig and b Tet at their IC50 concentrations for indicated time points. C Similarly, the graphs represent the relative viability of the combined treatment of oxaliplatin with a Tig and b Tet at their physiological concentrations for indicated time points. Percentage viability is presented as relative to non-treated controls, and significant differences in the viability between low and high metastatic cells were calculated using student’s t-test. *P<0.05**P<0.01 and ***P<0.001