Fig. 2
Effect of tigecycline/tetracycline on in vitro metastatic potential and spheroid formation. A-B In vitro metastatic potential was measured using transwell migration of CRC cells following treatment with Tig/Tet for 12 h. Cells migrated through trans-well membranes were stained with crystal violet and visualized under a microscope at total 100x magnification (100µm scale bar). A Representative images of migrated cells are shown. B Stained cells were counted and represented as relative migration units. C-D Cells were treated with Tig/Tet under low attachment conditions for 24h and allowed to form spheroids subsequently for the next 4–5 days. C Representative images of spheroids showing morphological changes on the 5th day were acquired under a bright-field microscope at total 100x magnification (100µm scale bar). D Viability of single cell suspension derived from spheroids after trypsnization and counting by trypan blue staining. Results are representative of three experimental replicates for each cell line, repeated at least 2–3 times, and values represent mean± SD. *P<0.05, **P<0.01