Fig. 3

E2F7 contributes to drug efflux and decreases DNA damage. (A-B) Flow cytometry assay (left panel) and statistical analysis of mean fluorescence intensity (MFI) (right panel) showing the uptake of Dox in cells with varying levels of E2F7 expression (n = 3, **p < 0.01). (C-D) RT-qPCR assay measuring the expression of ABCA2, ABCA3, ABCA8, ABCB1, ABCB4, ABCC3, ABCC4, ABCC9 and ABCG2 mRNA in the indicated cells (n = 3, *p < 0.05, **p < 0.01). (E) Western blot assay showing the expression of ABCA8 and ABCB4 protein in the indicated cells, GAPDH served as a loading control. (F) Western blot assay showing the expression of γH2A.X in the indicated groups with or without treatment of TMZ (200 µM for 48 h), β-Tubulin served as a loading control