Fig. 5

Hsa_circ_0005397 promotes the progression of HCC cell lines through EIF4A3. (A) Venn diagram analysis of the two databases (CircInteractome and CircFunBase) identified EIF4A3 as a candidate. (B) EIF4A3 expression in HCC tissues from TCGA datasets. (C) qRT-PCR detected the expression level of EIF4A3 in HCC tissues(n = 57). (D) The relative expression level of EIF4A3 in HCC cell lines. (E) Western Blot analysis of EIF4A3 in adjacent tissues and paired HCC tissues. (F) IHC staining of HCC tissues for EIF4A3 expression. ((magnification, 40x; scale bars, 100 μm). (G) Correlation between EIF4A3 and hsa_circ_0005397 mRNA expression in HCC tissues (n = 57). (H) qRT-PCR detected the expression of EIF4A3 in HCCLM3 cells transfected with shNC or shEIF4A3. (I) qRT-PCR analyzed the expression of hsa_circ_0005397 in HCCLM3 cells transfected with shNC or shEIF4A3. (J) RIP assay verified that EIF4A3 interacted with hsa_circ_0005397 and IgG was used as a control. (K) qRT-PCR detected the expression of hsa_circ_0005397 in HCCLM3 cells transfected with shNC + vector, shEIF4A3 + pcDNA or shEIF4A3 + pcDNA. (L) Cell proliferation ability was assessed by colony formation assays after HCCLM3 cells transfected with shNC + vector, shEIF4A3 + pcDNA or shEIF4A3 + pcDNA. Colony counts were counted to analyze colony formation rate, *P < 0.05, **p < 0.01, ***p < 0.001. (M) Cell migration and invasion abilities were determined by Transwell assays after HCCLM3 cells transfected with shNC + vector, shEIF4A3 + pcDNA or shEIF4A3 + pcDNA. (magnification, 20x; scale bars, 200 μm), *P < 0.05, **p < 0.01, ***p < 0.001