Fig. 1

The characteristics of hsa_circ_0005397 in hepatocellular carcinoma. (A) qRT-PCR detected the expression level of hsa_circ_0005397 in HCC tissues (n = 57). (B) The relative expression level of hsa_circ_0005397 in HCC tissues were normalized to normal tissues (n = 57). (C) Construction of an ROC curve for HCC patients based on hsa_circ_0005397 expression. (D) qRT-PCR analyzed the expression of hsa_circ_0005397 in cell lines. (*P < 0.05, **p < 0.01, ***p < 0.001). (E) The PCR product of qRT-PCR was run on 2% agarose gel, showing a single electrophoresis band of has_circ_0005397. (F) qRT-PCR analyzed relative expression after treatment with or without RNase R in BEL-7404. (G) qRT-PCR was used to measure the expression level of hsa_circ_0005397 in the nuclear and cytoplasmic. (H) qRT-PCR was used to detected the relative expression of hsa_circ_0005397 and RHOT1 mRNA in BEL-7404 cells treated with Actinomycin D at different time points