Fig. 8

SDC1 was essential for GBM proliferation. a, b Western blot and RT-qPCR were performed to assess the knockdown of SDC1 in U87 and U251 cells. ShNC represented the negative control group, shSDC1 represented the SDC1 knockdown group, and GAPDH was used as an internal reference. c CCK-8 assay was performed to assess cell viability in U87 and U251 cells at the indicated time points. The control group (shNC, blue line) and SDC1 knockdown group (shSDC1, red line) were compared for cell activity, as shown in the figure. d A clone formation experiment was conducted to measure the number of cell clones formed after 14 days in the control group (shNC) and SDC1 knockdown group (shSDC1) of U87 and U251 cells. The bar chart represented a statistical summary of cell colony numbers. e EdU incorporation experiment was conducted to detect the number of EdU-positive cells in the control group (shNC) and SDC1 knockdown group (shSDC1) of U87 and U251 cells. The bar chart was a statistical representation of the proportion of EdU-positive cells. Data was presented as Mean ± SD. Student’s t-test, *p < 0.05, **p < 0.01; ns, non-significant