Fig. 6

RNA-seq combined with ChIP-seq for the discovery of novel candidate genes. a Volcano plot of differential gene expression in U87 cells treated with NC (1% DMSO) and MZ1. The green and red dots represented all statistically significant genes with downregulated or upregulated expression (log2FoldChange < -1.0 or > 1, adjusted p-value < 0.05). b Enrichment analysis results of differentially expressed genes were performed using the GSEA Pathway Database to elucidate the functional role of MZ1 in gene regulation. c, d Using GO and KEGG pathway databases to analyze the cellular processes and signaling pathways involved in the downregulated genes after MZ1 treatment. e Enhancers were ranked based on the ascending H3K27Ac signal in U87 cells treated with NC (1% DMSO) and MZ1. The number of SE-regulated genes and the cutoff value of each group were shown in the figure. f The Venn diagram illustrated the intersection of downregulated genes in RNA-seq, genes regulated by SEs in the NC group, and genes unaffected by SEs regulation after MZ1 treatment. The right panel depicted a heatmap of the expression levels of these genes in the NC and MZ1 treatment groups