Fig. 3

MZ1 induced the GBM cell cycle G2 arrest and apoptosis. a The apoptosis ratio of cells after treatment with the concentrations of MZ1 as shown in the figure for 72 h, with 0 μM representing the final concentration of 1% DMSO treatment. b Western blot analysis was performed to assess the protein cleavage status of PARP after treatment with the concentrations of MZ1 as shown in the figure for 72 h, with 0 nM representing the final concentration of 1% DMSO treatment, using GAPDH as an internal reference. c The proportions of cell cycle phases after treatment with the concentrations of MZ1 as shown in the figure for 72 h, with 0 μM representing the final concentration of 1% DMSO treatment. d Real-time fluorescence quantitative PCR was used to measure the mRNA levels of Cyclin B and CDC2 after treatment with the concentrations of MZ1 as shown in the figure for 72 h, with 0 μM representing the final concentration of 1% DMSO treatment, using GAPDH as an internal reference. Data was presented as Mean ± SD. Student’s t-test, *p < 0.05, **p < 0.01; ns, non-significant