Fig. 2

MZ1 dose-dependently inhibited BRD4 protein and GBM cell proliferation. a Left panel: Cell viability of GBM cells after treatment with MZ1 at the indicated concentrations for 72 h, 0 μM corresponded to a final concentration of 1% DMSO. Green circles represented U87, red squares represented A172, yellow triangles represented U251, and purple inverted triangles represented LN229. Right panel: The IC50 value of MZ1 in different GBM cell lines. b Western blot analysis was performed to assess the protein levels of BRD2, BRD3, and BRD4 after treatment with MZ1 at the indicated concentrations for 48 h, 0 nM corresponded to a final concentration of 1% DMSO, using GAPDH as an internal reference. c CCK-8 assay was used to measure the changes in cell viability at different time points after treatment with various concentrations of MZ1, as shown in the figure. Red circles represented 1% DMSO treatment, and green squares represented the concentration of MZ1 treatment as depicted in the figure. d EdU incorporation experiment was conducted to detect the number of EdU-positive cells after 72 h of treatment with the concentrations of MZ1 as shown in the figure, with 0 μM representing the final concentration of 1% DMSO treatment. e A clone formation experiment was conducted to measure the number of cell clones formed after 14 days of treatment with the concentrations of MZ1 as shown in the figure, with 0 nM representing the final concentration of 1% DMSO treatment. Data was presented as Mean ± SD. Student’s t-test, *p < 0.05, **p < 0.01; ns, non-significant