Fig. 4

KIF23 was desuccinylated at K537 site. A The succinylation sites for KIF23 were predicted using the SuccinSite and GPSuc databases; B IP assay was used to analyze the succinylation sites of KIF23; C The HEK-293T cells transfected with an empty vector and SIRT7 overexpression vector were treated with CHX, then the protein expression of KIF23 was assayed by western blot at the different time points (0, 6, 12, and 24 h); D The quantitative analysis of KIF23 protein stability; E RNA stability assay was used to detect the existing KIF23 expression when actinomycin D treated at different time points (0, 6, 12, and 24 h); F IF ataining was performed to analyze the protein distribution of KIF23 and SIRT7 in HEK-293T cells. DAPI was used to stain the nuclei. KIF23, kinesin family member 23; IP, immunoprecipitation; HEK, human embryonic kidney; SIRT, sirtuin; CHX, cycloheximide; succ, succinylation; IF, immunofluorescence; DAPI, 4’,6-diamidino-2-phenylindole