Fig. 3

Silencing of SIRT7 increased KIF23-succ level in HEK-293T cells. A Genes positively and negatively related to KIF23 were obtained using the Linkedomics database; B The 80 genes positively related to the expression of KIF23. Red represents the genes associated with succinylation; C The levels of KIF23-succ in HTori3 and ATC cells (TPC1, 8305C, 8505C, BCPAP) were detected by Western blot; D Co-IP assay was used to screen the proteins associated with succinylation of KIF23 in HEK-293T cells; E Co-IP assay was used to detect the interaction between KIF23 and SIRT7 in HEK-293T cells; F Western blot was used to detect the levels of SIRT7, KIF23, and KIF23-succ after downregulation of SIRT7 in HEK-293T cells. KIF23, kinesin family member 23; ATC, anaplastic thyroid cancer; Co-IP, Co-immunoprecipitation; HEK, human embryonic kidney; SIRT, sirtuin; succ, succinylation