Fig. 3
Oridonin could triggered the ferroptosis in OS cells. A-C The expressions of ACSL4, SLC7A11, FTH1 and GPX4 in oridonin treated U2OS and 143B cells analyzed by WB and qRT-PCR, Actin and GAPDH as reference. (n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, vs. normal). D The accumulation of Fe2+ was detected in the oridonin treated cells by a microplate reader. (*p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, vs. normal). E-F ROS were detected by DCFH-DA probe in the oridonin treated cells, the accumulation of ROS was illustrated by green fluorescence (40X). Cells treated with ROSUP were used as positive control (*p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, vs. normal). G-H The mitochondrial membrane potential detected by JC-1 probe, dye Hoechest33342 was used for nuclear localization (100X). Cells treated with CCCP were used as positive control (*p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, vs. normal)