Fig. 2

Oridonin induced apoptosis in OS cells. A The enrichment analysis of KEGG pathways resulted in 162 pathways (p value < 0.05), select 20 of them to draw the signal path bar chart. B-C WB and qRT-PCR was used to analysis the expression levels of Bcl-2, Bax and cleaved caspase3 in 143B and U2OS cells exposed to oridonin (0–5-10 μM) for 24 h, Tublin and GAPDH as reference (n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, vs. normal). D-G The 143B and U2OS cells were treated with oridonin (0–10-20 μM) for 24 h, detected apoptotic cells by Annexin V-FITC and PI double staining and quantified (n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, vs. normal)