Fig. 4
From: Prognostic marker CD27 and its micro-environmental in multiple myeloma
The effect of CD27 on proliferation and apoptosis of myeloma cells. A, B CCK-8 was used to detect the proliferation of U266 and RPMI-8226 cells treated with sCD27 and 1F5 for 0, 24, 48, and 72 h. C, D Flow cytometry was used to analyze the apoptosis of U266 and RPMI-8226 cells treated with sCD27 and 1F5 for 0, 48, and 72 h. E qPCR detection of mRNA levels of PERK and ATF4 in MM cells treated with sCD27 and 1F5. F Western blotting detection of protein levels of PERK and ATF4 in MM cells treated with sCD27 and 1F5. G The correlation between ATF4 expression and OS of MM patients in GSE57317 was conducted by Kaplan–Meier analysis. * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001