Fig. 4

Identification of a 73-gene panel enriched in traffic-related structures and vesicle dynamics in PRNP^high samples and PRNP⁺ GBM cells. A Venn diagram of the 73 common genes with increased expression levels in PRNP^high GBM cells (TCGA bulk RNA-seq) and upregulated in PRNP⁺ cells (Darmanis et al., Neftel et al. and Richards et al.). B Over-representation analysis (gProfiler2 - GO, KEGG, Reactome, and WikiPathways) of common genes found in (A). C Schematic workflow of the analysis of an in-house cohort of GBM patient-derived samples. D Relative expression of PRNP, SYPL1, DSTN, ANXA1 and RAB31 in patient-derived GBM samples expressing Low (n=26) or High (n=26) PRNP levels by RT-qPCR and 2^-ΔΔCt, normalized by HPRT, GUSB and TBP. Student t-test, ****p<0.0001