Fig. 5

Twist overexpression in A498 cells basically eliminated the effect of sunitinib on SR-A498 cells. Determination of the proliferation ability of SR-A498 cells overexpressing Twist by CCK-8 assay (A). The results for the CCK-8 assay only mark the significance of 96 h drug treatment. Determination of the migration ability of SR-A498 cells overexpressing Twist by cell scratch. Representative images and statistical analysis (B). The migration (C) and invasion (D) abilities of cells were determined by Transwell assays. Key proteins related to proliferation, migration and invasion were detected by WB (F). Cell apoptosis was determined by flow cytometry (E). All experiments were repeated in triplicate in three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001