Fig. 4

Biological function of Twist in A498 cells in the presence/absence of sunitinib. The proliferation ability of cells was determined by CCK-8 assay (A). The figure only marks the significance of 96 h drug treatment. The migration ability of cells was determined by a cell scratch experiment (B). The migration (C) and invasion (D) abilities of cells were determined by Transwell assays. Cell apoptosis was determined by flow cytometry (E). Key proteins related to proliferation, migration and invasion were detected by WB (F). All representative images and statistical analyses are shown above. All experiments were repeated in triplicate in three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001