Fig. 3

RPARP-AS1 facilitated the proliferation and migration of OS cells. A The mRNA expression of RPARP-AS1 in five OS cell lines and the normal cell line. B MG63 or U2R cells were transfected with si-NC, si-RPARP-AS-1, si-RPARP-AS1-2, empty vector, or pcDNA3.1-RPARP-AS1 for 72 or 48 h. The efficiency of silencing or overexpression of RPARP-AS1 was assessed via qRT-PCR in MG63 or U2R cells, respectively. C Measurement of cell viability (MG63 and U2R cells) by CCK8 assay after silencing or overexpression of RPARP-AS1 for 72 or 48 h. D-F Results of cells colony formation, migration, and invasion assays after silencing or overexpression of RPARP-AS1 in MG63 or U2R cells, respectively. After 24 h of silencing or overexpression of RPARP-AS1, cells were resuspended and seeded into 6-well plates (1,000 cells/well) (D) or 12-well plates (8 × 10⁴ or 5 × 10⁴ cells/well) (E and F) for the indicated time. Scale bar, 100 μm. G Protein levels of RPARP-AS1, E-cadherin, N-cadherin, and vimentin were determined by Western blot analysis after silencing or overexpression of RPARP-AS1. H, I Cell apoptosis was assessed by flow cytometry (H) and Western blot analysis (I) after silencing or overexpression of RPARP-AS1 in MG63 or U2R cells. In B-F and H, a two-tailed unpaired Student’s t-test was used to determine statistical significance, with GAPDH or β-actin used as a loading control. Data are presented as mean ± SD (n = 3). *P < 0.05, **P < 0.01, compared to NC or Vector