Fig. 7

Changes of glutamate level in control, PMA, and PG72 samples of HL-60 cell line and RP-HPLC chromatograms. The trend of glutamate concentration after treatment with galectin-9 in the HL-60 cell line was decreasing in comparison to the PMA and control group but with no significant difference. (a.) Displays changes in glutamate concentration (µmol/L) in control, PMA, and PG72 groups in the HL-60 cell line. (b.), (c.), and (d.) Represents glutamate (GLU) in retention time of 8.15 min, 7.89 min, and 8.14 min for control, PMA, and PG72 groups, respectively. RP-HPLC method, OPA/MPA precolumn derivatization using fluorescence detector excitation 350 nm and emission 450 nm wavelengths. RP-HPLC was done on a C18 column (150 * 4.6 mm, 5 μm), using Mobile-phase A (1 g K2PO4 + 0.5 g KH2PO4 in 500 mL distilled water), and B (100 mL distilled water, 150 mL acetonitrile, and 300 mL isopropanol) with linear gradient from 10–100% B at a flow rate of 1.2 mL/min in 35 min