Fig. 5

mTORC and GLS proteins expression at 24 and 48 h after treatment with Gal-9 in THP-1 cell line. Western blot analysis confirmed the results of RT-qPCR for mTORC and GLS protein expression in the THP-1 cell lines. Based on diagrams (a., c.) and protein bands (b., d.), the expression of these proteins in the Gal-9 treated group compared to the control and PMA group had a significant increase at 24 h after Gal-9 treatment (PG24) (p = 0.0001) followed by a decrease at 48th hour. Asterisks represent statistical significance (* p < 0.05, ** p < 0.001, ***p < 0.0001). Full-length blots/gels of b-actin, mTOR and GLS in the THP-1 cell line are presented in Supplementary Fig. 1 (b.), Fig. 2 (b.) and Fig. 3 (b.), respectively. The grouping of blots cropped from different parts of different gels