Fig. 4

The mTORC and GLS proteins expression at 48 and 72 h after treatment with Gal-9 in the HL-60 cell line. Western blot analysis confirmed the results of RT-qPCR for mTORC and GLS protein expression in the HL-60 cell lines. Based on diagrams (a., c.) and protein bands (b., d.), the expression of these proteins in the Gal-9 treated group compared to the control and PMA group had an increasing trend with the highest level at 72 h after Gal-9 treatment (PG72) (p = 0.0001). Asterisks represent statistical significance (* p < 0.05, ** p < 0.001, ***p < 0.0001). Full-length blots/gels of b-actin, mTOR and GLS in the HL-60 cell line are presented in Supplementary Fig. 1 (a.), Fig. 2 (a.) and Fig. 3 (a.), respectively. The grouping of blots cropped from different parts of different gels