Fig. 7From: Metabolic subtypes and immune landscapes in esophageal squamous cell carcinoma: prognostic implications and potential for personalized therapiesMTHFD2 knockdown inhibited the proliferation and invasion of ESCA cells. A qRT-PCR assay to evaluate the transfection effect of MTHFD2 in TE-1 cells. B Western blot assay confirmed the transfection efficiency of MTHFD2 in TE-1 cells. C-E Functional experiments demonstrating the effects of downregulating MTHFD2 on (C) cell viability via CCK-8 assay, (D) the ability of ESCA cells to form colonies via clonogenic assay, and (E) promoting ESCA cell invasion via Transwell assay. **p < 0.01Back to article page