Fig. 4

Apoptosis detection and validation of the Wnt/β-catenin signal pathway. A, B Apoptosis levels of the different groups of ICC cells, as per Annexin V-EV450/7-AAD flow cytometry analysis. C-E Western blot analysis of different apoptosis-related proteins in FOXP1-overexpressed (HCCC-9810 and HUCCT1) cells and the control cells. F-G Western bolt of key proteins in the Wnt/β-catenin pathway. Tubulin was used as the loading control. Data are presented as means ± SD of three independent experiments. *p < 0.05. **p < 0.01, ***p < 0.001