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Fig. 4 | BMC Cancer

Fig. 4

From: Sequential gene expression analysis of myelodysplastic syndrome transformation identifies HOXB3 and HOXB7 as the novel targets for mesenchymal cells in disease

Fig. 4

Cell function analysis after blocking HOXB3 and HOXB7. (A) Interference efficiency of siRNA after cultured 48 h. (B) CCK-8 method to assess the effect of MSC-HOXB3 and MSC-HOXB7 on the proliferation in MSC. (C) Cell apoptosis analyzed after downregulating the expression of HOXB3 and HOXB7 by flow cytometry. (D) Oil Red O staining analyzed adipogenic differentiation after downregulating HOXB3 and HOXB7 MDS-MSC. (E) Alizarin Red staining analyzed osteogenic differentiation after downregulating HOXB3 and HOXB7 MDS-MSC. (F) The expression of hematopoiesis-related genes in MSC were analyzed by RT-PCR after downregulating HOXB3 and HOXB7 48 h. (G) CFU-colony forming of HSC were analyzed after co-incubation with MSC that downregulating the expression of HOXB3 and HOXB7. (* indicates significant difference, P < 0.05; scale bar: 100 μm)

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BMC Cancer

ISSN: 1471-2407

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