Fig. 3
BMI1 and ITGB1 are essential for the phenotypic modulation of OCSCC cells by CAF-EVs. A Differentially expressed genes in OCSCC cells treated with or without CAF-EVs filtered through RNA-sequencing. B The expression of five genes in OCSCC tissues and adjacent tissues from OCSCC patients was detected by RT-qPCR. C The expression of BMI1, ITGB1, and PXN-AS1 in the tumor tissues of OCSCC patients with or without lymph node metastasis. D The correlation between BMI1 and ITGB1 expression and OS in OCSCC patients was analyzed using a rank log-test. E The correlation between BMI1 and ITGB1 expression and RFS in OCSCC patients was analyzed using a rank log-test. F BMI1 and ITGB1 expression in CAF-EVs and the supernatant were measured using RT-qPCR. G CAL-27 and SCC-25 cells, with normal oral epithelial cells as control, was measured using RT-qPCR. H The expression of BMI1 and ITGB1 in CAL-27 and SCC-25 cells before and after CAF-EVs treatment was measured using RT-qPCR. I The protein expression of BMI1 and ITGB1 in normal oral epithelial cells and OCSCC cells was measured using western blot analysis. J The protein expression of BMI1 and ITGB1 in CAL-27 and SCC-25 cells before and after CAF-EVs treatment was measured using western blot analysis. K. The protein expression of BMI1 and ITGB1 carried by CAF-EVs was measured using western blot analysis. Paired or unpaired t-test, two-way ANOVA. Data are presented as means ± SD. Results are representative of at least three independent experiments (*p <.05)