Fig. 8

NUSAP1 overexpression promotes the proliferation, migration and invasion of PDAC cells. a mRNA expression levels of NUSAP1 in PANC-1 and CAPAN-1 cells were detected by reverse transcription quantitative PCR after transfection of lentiviral vectors containing NUSAP1 OE plasmids. b The protein expression level of NUSAP1 increased significantly after transfection with OE-NUSAP1. c The proliferation of PDAC cells was measured using CCK-8 assays. d Cell migration of CAPAN-1 cells in the NC and OE groups according to Wound healing assays. e Cell migration of PANC-1 and CAPAN-1 cells in the NC and OE groups according to Transwell assays. f Cell invasion of PANC-1 and CAPAN-1 cells in the NC and OE groups according to Transwell assays. Student’s t test was used for comparisons between groups. All experiments were performed in triplicate. *P < 0.05. NC, negative control; OE, overexpression