Fig. 4

Regulation of PTPRT on BIRC5 and its effect on tumour prognosis. A, B Overexpression of PTPRT led to decreased expression of BIRC5 (survivin) at protein level (P = 0.029, two-sided Student's t-test) in A549 (A) and H1975 (B) cells. Three replicates of Western blots were performed, full-length gels and blots were shown in Supplementary Fig. 1– 2. C Increased BIRC5 mRNA expression in si-PTPRT compared to si-NC cells. D Increased BIRC5 protein level in si-PTPRT compared to si-NC cells, full-length gels and blots were shown in Supplementary Fig. 3. E The upregulation of BIRC5 was associated with a worse prognosis for patients with LUAD. F Univariable and multivariable Cox regression analysis showed that BIRC5 was an independent risk factor of LUAD. G. The inverse correlation of PTPRT and BIRC5 in multiple cancers. Data were shown as the mean ± SD (n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 by unpaired Student’s t test