Fig. 2

Defining the cellular landscape of vulvar melanoma. (A) Distribution of UMI counts and gene counts before quality control. The vertical black lines indicating the QC filter criteria. (B) Uniform Manifold Approximation and Projection (UMAP) map showing the identified 12 cell clusters based on the transcriptomes of 12,243 cells. Cells are colored by Seurat clustering and annotated by cell types (each point represents a single-cell). (C) Process of subdivided melanocytes into two populations. (D). Proportion of cells from major cell types. (E) Violin plots showing markers in each cell cluster. (F) Heatmap presenting markers in all of the cell types. (G) Dotplots depicting the distribution of specific genes in cell landscape, namely EPHA3, PDGFB, PDGFRB, GNG2