Fig. 6

PA-induced KLF7/CCL2 pathway in BMA promoted the malignancy of PC-3 cells via GPR40/120. The GPR40 antagonist GW1100 was added to BMA cells under PA stimulation. The mRNA expression levels of KLF7/ CCL2 (A, B) and the secretion levels of CCL2 were determined (C).The protein expression levels of KLF7/ CCL2 were also determined (D, E). Next, the conditioned medium obtained from the BMA with PA + GW1100 (CM-BMA-PA + GW1100) was used for stimulating PC-3 cells. The proliferation (F), invasion (G), and migration (H) abilities of PC-3 cells were determined. In addition, The GPR120 antagonist AH7614 was added to BMA cells under PA stimulation. The mRNA expression levels of KLF7/ CCL2 (I, J) and the secretion levels of CCL2 were determined (K).The protein expression levels of KLF7/ CCL2 were also determined (L, M). Next, the conditioned medium obtained from the BMA with PA + AH7614 (CM-BMA-PA + AH7614) was used for stimulating PC-3 cells. The proliferation (N), invasion (O), and migration (P) abilities of PC-3 cells were determined. In the t-test, the differences with *P < 0.05, **P < 0.01, and ***P < 0.001 were statistically significant