Fig. 5

PA-induced KLF7/CCL2 pathway in BMA promoted the malignancy of PC-3 cells. A Co-transfection of the human CCL2 promoter region luciferase plasmid and the KLF7-overexpression plasmid into 293 T cells and the detection of the luciferase activity. The KLF7 overexpression plasmid was transfected into BMA cells. The mRNA and protein expression levels of KLF7/ CCL2 (B, C)and the secretion levels of CCL2 (D) were determined. Next, the conditioned medium obtained from the BMA with KLF7 overexpression (CM-BMA-AdKLF7) was used for stimulating the PC-3 cells. The proliferation (E), invasion (F), and migration (G) abilities of PC-3 cells were determined. Afterward, the small interfering RNA for KLF7 (si-KLF7) was transfected into BMA cells. The mRNA and protein expression levels of KLF7/CCL2 (H, I) and the secretion levels of CCL2 (J) were determined. Subsequently, the conditioned medium obtained from the BMA with si-KLF7 (CM-BMA-siKLF7) was used for stimulating PC-3 cells. The proliferation (K), invasion (L), and migration (M) abilities of PC-3 cells were determined. Finally, si-KLF7 was transfected into BMA cells under PA stimulation. The mRNA and protein expression levels of KLF7 and CCL2 were determined (N, O). The secretion levels of CCL2 were determined (P). Afterward, the conditioned medium obtained from the BMA with PA + si-KLF7 (CM-BMA-PA + siKLF7) was used for stimulating PC-3 cells. The proliferation (Q), invasion (R), and migration (S) abilities of PC-3 cells were determined. In the t-test and one-way ANOVA, the differences with *P < 0.05, **P < 0.01, and ***P < 0.001 were statistically significant